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Immuno-MALDI MS dataset for improved detection of HCVcoreAg in sera

机译:用于改善血清中HCVcoreAg检测的Immuno-MALDI MS数据集

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Complicated and large-scale challenge the contemporary biomedical community faces are development of highly-sensitive analytical methods for detection of protein markers associated with development of pathogenic mechanisms [2]. The atomic force microscopy (AFM) method in combination with specific fishing is unique among other analytical protein detection approaches; it allows visualization and counting of single protein molecules [3–6]. The present dataset focus on mass spectrometry method for detection of human hepatitis C virus core antigen (HCV core Ag) taking into account the potential modification with cations in blood serum samples, using mica chips for the atomic force microscopy (AFM-chips). To conduct specific protein fishing, we used flat AFM-chips preliminary sensibilized with molecular probes – aptamers, which are single-stranded DNA sequences. In our study we used four types of aptamers up to 85 nucleotides specific against the target protein – HCVcoreAg [3,4]. Working (n?=?19) and control (n?=?11) AFM-chips with aptamers were preliminarily immobilized on the surface in four zones and incubated in blood serum samples (See Supplementary fig. 1). Analysis of MS data regarding modification of marker protein peptides with?Na+, K+, K2Cl+, and Na2Cl?+?ions enables to enhance the reliability of target proteins detection in the serum thereby demonstrating a high diagnostic potential.
机译:当代生物医学界面临的复杂且大规模的挑战是发展高度灵敏的分析方法,以检测与致病机制发展有关的蛋白质标记[2]。原子力显微镜(AFM)方法与特定的捕捞方法相结合在其他分析性蛋白质检测方法中是独一无二的。它允许可视化和计数单个蛋白质分子[3-6]。考虑到血清样品中阳离子的潜在修饰,目前的数据集中在质谱法上,用于检测人类丙型肝炎病毒核心抗原(HCV核心Ag),使用云母芯片进行原子力显微镜检查(AFM芯片)。为了进行特定的蛋白质捕捞,我们使用了预先被分子探针-适体(aptapter)敏化的扁平AFM芯片,它们是单链DNA序列。在我们的研究中,我们使用了四种类型的适体,它们针对靶蛋白HCVcoreAg的特异性最高为85个核苷酸[3,4]。将带有适体的AFM工作芯片(n≥19)和对照(n≥11)预先固定在四个区域的表面上,并在血清样品中孵育(参见补充图1)。对有关用ΔNa+,K +,K 2 Cl +和Na 2 Cl 2 +离子修饰标记蛋白肽的MS数据进行分析,可以提高血清中靶蛋白检测的可靠性,从而显示出很高的诊断潜力。

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