Effects of sodium selenite administration during diethylnitrosamine intoxication in rats

摘要

The aim of this study was to evaluate the toxicity of a single dose of 100 mg/kg body weight of NDEA and to compare the effects of selenium administration before and after the NDEA dose (pre- vs. post-treatment), on the evolution of some biochemical parameters. Male Wistar rats were divided into five groups (n=5): control group, NDEA group (NDEA: 100 mg/kg bw by gavage, single dose), NDEA + Se3 group (NDEA: 100 mg/kg bw by gavage, single dose + Se+4: 3 mg/kg bw by gavage, in the first four days), Se group (Se+4: 3 mg/kg bw by gavage, for four days) and Se3 + NDEA group (Se+4: 3 mg/kg bw by gavage, for four days before NDEA administration + NDEA: 100 mg/kg bw by gavage, single dose). The animals were sacrificed after ten days from NDEA administration and blood was collected. The biochemical parameters were determined using a RX Imola automatic analyzer. In the NDEA group uric acid, total bilirubin, direct bilirubin, HDL-cholesterol, LDL-cholesterol levels and ALT and AST activities were significantly increased compared to the control group. Total bilirubin, direct bilirubin, HDL-cholesterol and iron blood levels, as well as GGT and AST activities were significantly increased in NDEA+Se group, compared to the Se+NDEA group, whereas LDL-cholesterol levels and ALT activities were significantly increased in Se+NDEA group, compared to the NDEA+Se group. At this dose and observational period, NDEA was slightly toxic. The post-treatment with sodium selenite increased NDEA toxicity and was more severe than the pre-treatment.