Functional Analysis of Dendritic Cells Generated from T-iPSCs from CD4+ T Cell Clones of Sj?gren's Syndrome

摘要

Summary Although it is important to clarify the pathogenic functions of T?cells in human samples, their examination is often limited due to difficulty in obtaining sufficient numbers of dendritic cells (DCs), used as antigen-presenting cells, especially in autoimmune diseases. We describe the generation of {DCs} from induced pluripotent stem cells derived from T?cells (T-iPSCs). We reprogrammed CD4+ T?cell clones from a patient with Sj?gren's syndrome (SS) into iPSCs, which were differentiated into {DCs} (T-iPS-DCs). T-iPS-DCs had dendritic cell-like morphology, and expressed CD11c, HLA-DR, CD80, CD86, and also BDCA-3. Compared with monocyte-derived DCs, the capacity for antigen processing was similar, and T-iPS-DCs induced the proliferative response of autoreactive CD4+ T?cells. Moreover, we could evaluate T?cell functions of the patient with SS. In conclusion, we obtained adequate numbers of {DCs} from T-iPSCs, which could be used to characterize pathogenic T?cells in autoimmune diseases such as SS.