Morphology of Fresh and Cryopreserved Sperms of Banana Shrimp Penaeus merguiensis (De Man, 1888)

摘要

The spermatophore morphology of the P. merguiensis from Kedah, Malaysia is described. About 10cryopreserved groups as 6, 12 and 24 h, 7, 30, 60, 90, 120, 150 and 180 days and fresh as control was examinedfrom each of three replication were evaluated for sperm gross morphology evaluations. A fully mature male?sbroodstock of P. merguiensis was taken with fresh spermatophores and evaluated for sperm morphologically.Cryopreserved spermatophore after the thawing 27°C/2 min (fresh and frozen) individually transferred into glasshomogenizer (High speed variable speed reversible, Glas-col, Terre Havte In USA) with 200 μL of Ca-F saline.Fixation, dehydration by series of alcohol, Critical Point Dry (CPD) and mount specimens on to stubs using orcarbon dots as well as using Auto Fine Coater and Sputter Coater moreover scanning by Model JEOL 6360LAscanning electron microscope. The cryopreserved spermatophore shows similarities with those of fresh, therewere no significant differences (p>0.05) between the freshly spermatophore and spermatophorestored up to90 days at -196°C liquid nitrogen.