ANTIMICROBIAL ACTIVITY OF THE METHANOLIC EXTRACT AND COMPOUNDS FROM THE STEM BARK OF GARCINIA LUCIDA VESQUE (CLUSIACEAE)

摘要

We. investigated. hereby. the. methanolic. crude. extract.of. the. stem.bark.of. Garcinia. lucida.Vesque..The.genus. Garcinia.(Clusiaceae.or.Guttiferae).consists. of. about. 200. species. mainly. encountered. in. lowland. rain. forests. of. the. tropical. world,. particularly. in. Africa. and. Southeast. Asia1 ,2.. 21.Garcinia.species.are.represented.in.Cameroon.among.which. Garcinia.lucida.vesque3..Dichloromethane.sub‐fraction.from.the.methanol.crude.extract.of.the.stem.bark.of.Garcinia.lucida.Vesque.afforded.eleven.compounds.namely.putranjivic.acid. 1,.methyl.putranjivate.3,.their.intermediary.lactone.2,.friedeline. 4,.cycloartenol. 5,.1,2‐dihydroxy‐.xanthone. 6,.1‐hydroxy‐2‐methoxyxanthone. 7,.betulinic.acid. 8,.oleanolic..acid. 9,. β‐sitosterol. 10.and.stigmasterol. 11..Except. 5,. all.those.compounds. are. reported. for.the.first.time.from. Garcinia. lucida.. The.structures. of.isolated. compounds. were.established.on.the.basis.of.1D/2D.NMR.experiments,.mass.spectrometric.and.by.comparison.of.data.with.those.of.the.literature..The.methanol.crude.extract.and.pure.compounds.from.CH2Cl2. fraction. were. tested. for. their. antimicrobial. activity. against. Escherichia. coli,.Pseudomonas.aeruginosa,.Salmonella.typhi,.Staphylococcus.aureus,.and.Candida.albicans..The.crude.extract.showed.good.inhibitory.potential.with.a.MIC.value.of.64. μg/mL.on. Candida. albicans. while.pure.compounds.were.poorly.active..Only.compound.5.exhibited.moderate.and.selected