A Penicillin Biosensor by Using Silver Nanoparticles

摘要

Based enzymatic biosensor for selective detect of penicillin by using silver nanoparticles designed.Convergence of bioelectrochemistry and nanotechnology improved duty of novel biosensors. Heresilver nanoparticles used to facilitate electron exchange between biosensor elements and investigatedwith XRD, TEM and size distribution techniques and the mean size of the silver nanoparticles was1+ around 90 10 nm. Main equation for this research was: Penicillin + H2O = penicilloate + H . Theelectrochemical evaluations applying the potentiometric method were carried out for differentpenicillin G salt concentrations varying from 100 M to 100 mM made in a buffer solution at pH 7.4.The tested sensor configuration showed a wide linear dynamic range for the output response vs. thelogarithmic concentration of penicillin G salt solution. To investigate the reproducibility of the givensensor, we examined the potentiometric reply of all five sensor electrodes in 5, 10, 20 and 30 mMpenicillin solutions also the relative standard deviation was detected to be less than 4%.