Screening of Cellulase Production by Fungal Isolates from Rhizosphere Region of Mine Degraded Land in Dalli-Rajhara (Chhattishgarh)

摘要

The main objective of this study is to screen potential offungi which was able to secret cellualse enzyme, maximize economicbenefits and at the same time protection of environment by microbialactivity for restoration of degraded land. Cellulase are group ofhydrolytic enzymes capable of hydrolyzing the most abundantorganic polymer i.e. Cellulose to smaller sugar components includingglucose subunits. Quantitative method was used for screening screenpotential of fungi. After 5 days of incubation clear zone aroundfungal colonies were able to observe with the help of stain Congored. Out of 38 test fungi 15 was able to give clear zone. Highest clearzone diameter was observed in Periconia hispidula (1.5cm), Mucorhiemalis (1cm) and minimum was observed in Alternaria alternata(0.27) and Cladosporium oxysporum (0.28cm). Screened fungi wereused as a biofertilizer, for increasing soil fertility and restored thedegraded land.