Increased Longevity of Hematopoiesis in Continuous Marrow Cultures and Radiation Resistance of Marrow Stromal and Hematopoietic Progenitor Cells from Caspase-1 Homozygous Recombinant-negative (Knockout) Mice

摘要

Aim: We determined whether absence of caspase-1 altered the stress response of hematopoietic and bone marrow stromal cells in vitro. Materials and Methods: Long-term bone marrow cultures from caspase-1 -/- and control caspase-1 +/+ mice were established and the derived bone marrow stromal and interleukin-3 (Il-3)-dependent hematopoietic progenitor cell lines were evaluated for radiosensitivity. Results: Long-term bone marrow cultures from caspase-1 -/- mice generated hematopoietic cells for over 30 weeks in vitro, significantly longer than controls did (p=0.0018). Bone marrow stromal (mesenchymal stem cell) and Il-3-dependent hematopoietic progenitor cell lines from caspase-1-/- marrow cultures compared to caspase-1 +/+ were radioresistant (p=0.0486 and p=0.0235 respectively). Total-body irradiated caspase-1 -/- mice were not significantly radioresistant compared to controls (p=0.6542). Conclusion: Caspase-1 deletion increases hematopoiesis and radioresistance of bone marrow cells in vitro.