首页> 外文期刊>Annals of microbiology >Sequence analysis of 16S rRNA gene and 16S–23S rRNA gene intergenic spacer region for differentiation of probioticsLactobacillus strains isolated from the gastrointestinal tract of chicken
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Sequence analysis of 16S rRNA gene and 16S–23S rRNA gene intergenic spacer region for differentiation of probioticsLactobacillus strains isolated from the gastrointestinal tract of chicken

机译:16S rRNA基因和16S–23S rRNA基因间间隔区的序列分析,用于区分益生菌从鸡胃肠道分离的乳酸杆菌菌株

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Twelve probioticLactobacillus strains which were previously identified with classical biochemical tests were re-identified using molecular methods. Comparative sequence analyses of the 16S rRNA gene and 16S–23S rRNA gene intergenic spacer region (ISR) were applied. Results of the study showed that mis-identification at species level occurred at high rate when classical biochemical tests were used. Nine of the strains showed discrepancy in their identity. These nine strains which were previously identified through biochemical tests asL. brevis C1,L. brevis C10,L. fermentum C16,L. brevis C17,L. crispatus I12,L. acidophilus I16,L. fermentum I24,L. fermentum I25 andL. acidophilus I26 were re-identified asL. reuteri C1,L. reuteri C10,L. reuteri C16,L. panis C17,L. brevis I12,L. gallinarum I16,L. salivarius I24,L. brevis I25 andL. gallinarum I26, respectively, using 16S rRNA gene and 16S–23S rRNA gene ISR analysis.Lactobacillus strains I16 and I26 initially could not be classified into a single taxon by 16S rRNA gene sequencing but the identities of these two strains were eventually resolved by 16S–23S rRNA gene ISR sequence analysis asL. gallinarum. Sequence analysis of 16S rRNA gene in complementary with 16S–23S rRNA gene ISR could be potentially useful for rapid and reliable identification of bacteria.
机译:使用分子方法重新鉴定了十二个以前通过经典生化测试鉴定的益生菌乳酸杆菌菌株。应用了16S rRNA基因和16S-23S rRNA基因基因间隔区(ISR)的比较序列分析。研究结果表明,当使用经典的生化试验时,物种水平上的错误识别率很高。九株菌株的身份差异。这九种菌株以前通过生化试验鉴定为L。布雷维斯C1,L。布雷维斯C10,L。发酵菌C16,L。布雷维斯C17,L。脆皮I12,L。嗜酸性I16,L。发酵I24,L。 I25和L.嗜酸性I26被重新标识为L。路透社C1,L。罗伊特里C10,L。罗伊特里C16,L。潘尼斯C17,L。布雷维斯I12,L。鸡胆I16,L。唾液I24,L。 brevis I25和L。分别用16S rRNA基因和16S-23S rRNA基因ISR分析。 23S rRNA基因ISR序列分析为L。鸡油菌。与16S–23S rRNA基因ISR互补的16S rRNA基因的序列分析可能对快速,可靠地鉴定细菌有用。

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