The Analysis of the Genotype Segregation at Ssr Loci among Double-Cross F1 Population Resistant to Northern Corn Leaf Blight and Head Smut in Maize

摘要

Abstract: With the help of previously reported linked-markers to resistance genes Ht1, Ht2, Ht3, HtN1 and QTLs to northern corn leaf blight (NCLB) and resistance QTLs to head smut, total 94 tightly linked SSR markers falling in or nearby the reported markers were chosen from the public database of maize genome to conduct this study. Ten (10) SSR markers showing polymorphisms among 3 parents (one resistant to Exserohilum turcicum, one resistant to head smut and one susceptible to both of them) were used to detect 32 double-cross F plants. 1 The analysis of the genotypes segregation at the SSR loci showed parental preference toward Ent12 with resistance to NCLB. In addition to 4 kinds of band patterns of normal genotype, the double-cross F population 1 also produced abnormal genotype bands same as Parents Ent17, Ent12 or three parental hybrid bands, respectively. These results are valuable reference for the pyramiding multi-resistance genes to both Exserohilum turcicum and head smut and the application of double-cross F plants in maize breeding.Key words: Maize % Double-Cross F Population % SSR Marker % Genotype Segregation 1