THE POTENTIAL OF CROSS RESISTANCE BETWEEN MONO TRAIT AND STACKED GENE TRANSGENIC EVENTS - A CASE STUDY

摘要

Medicinal plants preparations are still the most popular life saving remedies for a larger part of the world population.However, exhaustion of natural resources demands new approaches in utilization and protection of these species.Biotechnology may be a powerful tool for enhancing the productivity of novel secondary metabolites. In recent yearsAgrobacterium mediated plant transformation, due to its simplicity and efficiency, has become quite used method for theintroduction of foreign genes into plant cells followed by regeneration of genetically improved plants. The approach wassuccessful in a number of technical and food crops but it is still limited in medicinal plants. One of the most appropriatemethods for medicinal plants engineering is genetic transformation leading to increased synthesis of biological activesubstances in root cultures or in regenerated plantlets.Rhodiola rosea is a medicinal plant with limited area of distribution. Roots and rhizomes contain biological activesubstances used in prevention and treatment of socially important diseases.This article illustrates the possibilities of biotechnology for regeneration of Golden root and for genetic transformationaiming at stimulation of secondary metabolite production in vitro. Efficient system for propagation in vitro wasdeveloped using leaf nodes explants and was used for the genetic transformation experiments. Agrobacterium rhizogenesATCC 15834 was used for hairy roots induction. Various factors (like density of bacterial suspension, co-cultivation timeduration, concentration of the selective antibiotic) with impact on the efficiency of the transformation procedures werestudied. Three transformation methods were compared: (i) spilling drops of bacterial suspension on the explants; (ii)injuring explants and dipping them into the bacterial suspension; (iii) injecting plantlets. Favorable and frustratingconditions were observed and discussed.