首页> 外文期刊>African Journal of Biotechnology >Characterization of Indian and exotic quality protein maize (QPM) and normal maize (Zea mays L.) inbreds using simple sequence repeat (SSR) markers
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Characterization of Indian and exotic quality protein maize (QPM) and normal maize (Zea mays L.) inbreds using simple sequence repeat (SSR) markers

机译:使用简单序列重复(SSR)标记表征印度和外来优质蛋白玉米(QPM)和普通玉米(Zea mays L.)近交种

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Polymorphism analysis and genetic diversity of normal maize and quality protein maize (QPM) inbreds among locally well adapted germplasm is a prerequisite for hybrid maize breeding program. The diversity analyses of 48 maize accessions including Indian and exotic germplasm using 75?simple sequence repeat (SSR)markers yielded 258 scorable alleles, out of which 251 alleles were polymorphic with an average of 3.35 alleles per locus. The polymorphism information content (PIC) values of all the polymorphic primers across the maize genotypes varied from 0.11 to 0.91, with an average value of 0.56. Di-nucleated repeats showed more number of average alleles (4.2) with more mean PIC value (0.61) than tri-, tetra-, penta- and hexa- nucleotide repeats. Gene diversity (He) was in the range of 0.11 (umc1766) to 0.81 (mmc0371 and?bnlg1600) with an average value of 0.59, while heterozygosity (Ho) was observed with an average of 0.19, ranging from 0.02 (umc1495) to 0.98 (umc1906). Inbreeding coefficient (F) varied from 0.06 (umc2075) to 1.00 (15 SSR loci). Thus,?the present study resulted to the identification of highly polymorphic SSR loci?mmc0371,?umc2364,?umc1568,?bnlg1600,?phi026,?umc2071 and?bnlg1904?by considering the parameters of PIC value (≥0.74), gene diversity (≥0.75), inbreeding coefficient (≥0.62) and polymorphic alleles (≥4). These 7 polymorphic primers can be effectively used in a molecular breeding programs and?quantitative trait loci (QTL)mapping studies since they exhibited very high polymorphism over other loci. Genotype pairs VQL 2 and CML 173 was observed to serve as ideal parents for mapping modifiers since they differ significantly for tryptophan content and also showed lowest similarity of 27% between them. Among all the genotypes,?V 370 and B 06-7 (22%), V 25 and CM 152 (22%) and V 341 and CM 145 (24%) genotype pairs can be used in maize hybrid breeding programs for producing high yielding hybrids.
机译:普通玉米和优质蛋白玉米(QPM)近交种在本地适应性强的种质上的多态性分析和遗传多样性是杂交玉米育种计划的前提。利用75个简单重复序列(SSR)标记对包括印度和外来种质在内的48个玉米种质进行了多样性分析,得到了258个可等位基因,其中251个等位基因为多态性,每个基因座平均为3.35个等位基因。玉米基因型上所有多态性引物的多态性信息含量(PIC)值在0.11至0.91之间变化,平均值为0.56。与三,四,五和六核苷酸重复序列相比,双核重复序列显示更多的平均等位基因(4.2)和更多的平均PIC值(0.61)。基因多样性(He)在0.11(umc1766)至0.81(mmc0371和?bnlg1600)的范围内,平均值为0.59,而杂合度(Ho)的平均值为0.19,范围在0.02(umc1495)至0.98之间(umc1906)。近交系数(F)从0.06(umc2075)到1.00(15个SSR位点)不等。因此,本研究通过考虑PIC值(≥0.74),基因多样性的参数,鉴定出高度多态的SSR基因座——mmc0371,?umc2364,?umc1568,?bnlg1600,?phi026,?umc2071和?bnlg1904。 (≥0.75),近交系数(≥0.62)和多态等位基因(≥4)。这7种多态性引物可以有效地用于分子育种程序和定量性状基因座(QTL)定位研究,因为它们与其他基因座相比具有很高的多态性。观察到基因型对VQL 2和CML 173是作图修饰子的理想亲本,因为它们的色氨酸含量差异显着,并且它们之间的相似性最低,为27%。在所有基因型中,ΔV370和B 06-7(22%),V 25和CM 152(22%)以及V 341和CM 145(24%)基因型对可用于玉米杂交育种计划,以产生高产生杂种。

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