Assessment of genetic relationships among Spring Dendrobium cultivars and varietal materials using amplified fragment length polymorphism (AFLP) analysis

摘要

(AFLP) markers with near-infrared fluorescence-labeled primers. Eight EcoRI + 3 bases/MseI + 3 bases primer set combinations were used in this investigation. Each selected primer set generated 113 to 158 scorable fragments. A total of 1102 AFLP fragments were detected, of which 778 were polymorphic (70.6%). An unweighted pair-group method of the arithmetic averages (UPGMA), principal coordinate analysis (PCOA), and bootstrap analysis were used to analyze the genetic relationships. The 30 cultivars were separated into five clusters. Cluster I contains 6 cultivars that are either from Senlan No.1 or Senlan No. 6 with Jaccard’s similarity coefficients ranging from 0.70 to 0.80. All of these 6 cultivars came from Taiwan, and were derived from somaclonal variants or sports. Just 3 cultivars were positioned in cluster II ranging from 0.71 to 0.76, and also originated from Taiwan. Cluster III included 13 cultivars, Jaccard’s similarity coefficients varied from 0.69 to 0.84. Seven cultivars from Senlan No. 15 or ‘Snowboy Romance’ were situated in cluster IV with Jaccard’s similarity coefficients ranging from 0.69 to 0.82. Only?‘Santana Canary’ was positioned in cluster?Ⅴ?with Jaccard’s similarity coefficient at 0.62. This study established the genetic relationships of these commonly cultivated Spring?Dendrobiums, and raised a concern over genetic vulnerability of cultivars in this study because of their close genetic similarities.