Physicochemical Properties of Bovine Viral Dirrhoea Virus of an Indian Isolate

摘要

Study of physicochemical properties of BVDV revealed that virus can be inactivated at 56oC for 30 min and by the treatment of 1% NaOH, 5% chloroform and 0.05% trypsin for an hour exposure. Ammonium chloride at 10mM concentration completely inhibited the penetration of virus in Sheep Fetal Thymus Riems (SFTR) cell line when treated for 15 min. Methyl blue dye up to 10μM had no effect on BVDV in concentration, however, crystal violet inactivated the virus completely at the same concentration, both in presence and absence of organic matter (milk powder). Acidic pH (≤3) and disulphide bond reducing agent also inactivated BVDV after 1 h exposure. Ethanol was effective at concentrations of 70, 80, and 95 percent in inactivating the virus after 15 sec of exposure at 20oC, whereas propanol inactivated the virus in same time at 30 and 45 percent concentrations. During the past few years, many healthcare workers have changed from antimicrobial liquid soaps to alcohol–based hand rubs for post–contamination treatment of hands as suggested by the CDC guideline for hand hygiene. As BVDV serves as a surrogate model for Hepatitis C virus (HCV), the present study can contribute to evaluate and devise effective methods of controlling the latter