首页> 外文期刊>Acta Ichthyologica et Piscatoria: an international journal for ichthyology and fisheries >Qin F., Wang L., Liu S., Wang Z. 2013. Characterization of reference genes in rare minnow, Gobiocypris rarus (Actinopterygii: Cypriniformes: Cyprinidae), in early postembryonic development and in response to EDCs treatment. Acta Ichthyol. Piscat.  43 (2): 127–137.
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Qin F., Wang L., Liu S., Wang Z. 2013. Characterization of reference genes in rare minnow, Gobiocypris rarus (Actinopterygii: Cypriniformes: Cyprinidae), in early postembryonic development and in response to EDCs treatment. Acta Ichthyol. Piscat.  43 (2): 127–137.

机译:秦峰,王凌,刘珊,王Z。2013。胚胎后早期发育和对EDCs处理中的稀有小now鱼,拟南芥(Actinopterygii:Cypriniformes:Cyprinidae)参考基因的表征。乙酰甲酚。 Piscat。 43(2):127–137。

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Background. Endocrine disrupting chemicals (EDCs) are natural and anthropogenic compounds discharged into the environment known to disrupt the endocrine system of humans and animals by mimicking functions of steroids in vivo. Many important events occurring during early postembryonic development, in relation to the gene expression attracted our attention. Quantitative real-time PCR (qRT-PCR) is a sensitive and highly reproducible method for gene expression analysis, with gene expression levels quantified by normalization to reference gene. The aim of this study was to select the suitable reference gene after EDCs exposure and during early postembryonic development.
机译:背景。内分泌干​​扰化学物质(EDC)是天然的和人为产生的化合物,排放到环境中,通过模仿类固醇的体内功能破坏人类和动物的内分泌系统。与基因表达有关的胚胎后早期发育过程中发生的许多重要事件引起了我们的注意。实时定量PCR(qRT-PCR)是一种灵敏且高度可重复的基因表达分析方法,其基因表达水平可通过对参考基因进行标准化来量化。这项研究的目的是在EDCs暴露后和胚胎后早期发育过程中选择合适的参考基因。

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