Production of Granulocyte-Macrophage Colony-Stimulating Factors (GM-CSF) by Various Mammalian Cell Lines Cultured in a Protein-Free Synthetic Medium

摘要

References(25) Cited-By(3) Thirteen cell lines, all of which had been adapted to grow in a chemically defined medium, produced colony-stimulating factor (CSF), but the production rates of the factor varied considerably among the cell lines. Rat liver parenchymal, monkey kidney, and rat spleen plasma cell lines were comparable to L-P3 cells in their CSF-producing activity. Isoelectrofocusing and gel-filtration chromatography in the presence of 6 M guanidine HC1 showed that the CSFs of the liver and kidney cell lines resembled L-P3 CSF, whereas the CSF of spleen cells differed from the other CSFs in its molecular properties. In addition, the CSF of the spleen cell line predominantly stimu-lated the formation of granulocytic colonies, but the L-P3 CSF and other CSFs mainly enhanced monocytic colony formation.