Performance and community structure change of immobilized marine petroleum-degrading bacteria in the degradation process

摘要

In order to further discuss the effect of diesel degradation and the biological diversity change in degradation process, the biodegradation effect and microbiological characteristics of marine immobilized petroleum-degrading bacteria were analyzed. In this paper, the biodegradations of diesel by petroleum-degrading bacteria immobilized on corn straw and free bacteria were compared. The diesel degradation rate of immobilized petroleum-degrading bacteria was up to 66% in artificial seawater on the 30th day, which was far higher than that of free petroleum-degrading bacteria (only 20%). The kinetics of diesel biodegradation of immobilized petroleum-degrading bacteria and free bacteria were also studied. The diesel degradation rate of diesel by immobilized petroleum-degrading bacteria is 3.5 times of that by free petroleum-degrading bacteria. Additionally, nutrients (e.g., nitrogen and phosphorus) released from corn straw were analyzed. The results indicated that a certain amount of nutrients released from corn straw plays a certain role in promoting the degradation of diesel by immobilized petroleum-degrading bacteria. The analysis of the microbial community structure showed that, although the population was changed in the degradation process, the dominant bacterial community was still composed of the Pseudomonas sp., Stenotrophomonas sp., Achromobacter sp., and Alcanivorax sp.