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Benzene degradation with bacterial strains isolated from rhizosphere of Cannabis sativa being irrigated with petroleum refinery wastewater

机译:用石油炼厂废水灌溉从大麻根际分离的细菌菌株降解苯

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Rhizoremediation is considered as an environmentally friendly technique to deal with the contamination of petroleum hydrocarbons. The current study was aimed at checking the potential of indigenous micro-organisms isolated from rhizosphere soil being irrigated by the wastewater of petroleum refinery to degrade benzene efficiently. For this study, soil samples were taken from the discharge point of oil refinery located in Rawalpindi, Pakistan, by brushing off the soil from roots of Cannabis sativa. The soil was processed for isolation of indigenous microbial strains. Sixteen bacterial strains isolated from the soil were screened for their potential to grow using benzene as sole carbon source in vitro. These were exposed initially to 250 mg L-1 followed by 500 and 750 mg L-1 of benzene, where only seven strains were able to survive with optical density (OD600) of >= 1.1. Three better performing strains with OD600 >1.6 were further subjected to degrade 1,000 mg L-1 benzene (sole carbon source) using M9 media. The community diversity analysis for the best performing strains was carried out using 16S ribosomal RNA. Pseudomonas aeruginosa (SAR-1) was the best degrader with 85% benzene degradation in 72 hours followed by Bacillus cereus (SAR-2) and Acinetobacter junii (SAR-3) with 83 and 70% efficiency, respectively. To the best of our knowledge, A. junii (SAR-3) is being reported as a benzene degrader for the first time.
机译:根治技术被认为是对付石油碳氢化合物污染的环保技术。当前的研究旨在检查从根际土壤中分离的本土微生物的潜力,这些微生物被炼油厂的废水灌溉以有效降解苯。在这项研究中,从位于巴基斯坦拉瓦尔品第的炼油厂的排放点采集了土壤样品,方法是从大麻根部刷掉土壤。对土壤进行处理以分离出本地微生物菌株。使用苯作为唯一的碳源,从土壤中分离出的16种细菌菌株具有潜在的生长潜力。首先将它们暴露于250 mg L-1,然后暴露于500和750 mg L-1的苯中,其中只有7个菌株能够以> = 1.1的光密度(OD600)存活。使用M9培养基,将OD600> 1.6的三种性能更好的菌株进一步降解为1,000 mg L-1苯(唯一碳源)。使用16S核糖体RNA对性能最佳的菌株进行了群落多样性分析。铜绿假单胞菌(SAR-1)在72小时内降解率最高,苯降解率达85%,其次是蜡状芽孢杆菌(SAR-2)和不动杆菌(SAR-3),效率分别为83%和70%。据我们所知,A。junii(SAR-3)首次被报道为苯降解剂。

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