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Cytotoxicity and induction of DNA double-strand breaks by components leached from dental composites in primary human gingival fibroblasts

机译:从原代人牙龈成纤维细胞中牙科复合材料浸出的成分的细胞毒性和DNA双链断裂的诱导

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摘要

Introduction. The public interest steadily increases in the biological adverse effects caused by components released from resin-based dental restorations. Objective. In this study, the cytotoxicity and the genotoxicity were investigated of following released components from dental resin restorations in human gingival fibroblasts (HGF): tetraethyleneglycol dimethacrylate (TEEGDMA), neopentylglycol dimethacrylate (Neopen), diphenyliodoniumchloride (DPIC), triphenyl-stibane (TPSB) and triphenylphosphane (TPP). Methods. XTT based cell viability assay was used for cytotoxicity screening of substances. 7-H2AX assay was used for genotoxicity screening. In the 7-H2AX assay, HGFs were exposed to the substances for 6h. Induced foci represent double DNA strand breaks (DSBs), which can induce ATM-dependent phosphorylation of the histone H_2AX. Cell death effects (apoptosis and necrosis), induced by the substances were visually tested by the same investigator using the fluorescent microscope. Results. All tested substances induced a dose-dependent loss of viability in HGFs. Following toxicity ranking among the substances at ECso-concentration were found in the XTT assay (mM, mean ± SEM; n = 5): DPIC>Neopen>TPSB>TPP>TEEGDMA. DSB-foci per HGF-cell were obtained, when HGFs were exposed to the EC_50-concentration of each substance in the following order (mean ± SEM; n = 3): DPIC > Neopen > TPSB > TPP > TEEGDMA. Multi-foci cells (cells that contain more than 40 foci each) in 80 HGF-cells at ECso-concentration of each substance were found as follow (mean ± SEM; n = 3): DPIC > Neopen > TPP > TPSB > TEEGDMA. Cell apoptosis contained in each substance at ECso-concentration in the following order (mean ± SEM; n = 3): DPIC > Neopen > TPSB > TPP >TEEGDMA. Cell necrosis contained in each substance at EC5o-concentration in the following order (mean ± SEM; n = 3): DPIC > Neopen > TPSB > TPP > TEEGDMA. Conclusion. Leached components from dental resin restorations can induce DNA DSBs and cell death effects in HGFs.
机译:介绍。从树脂基牙齿修复物中释放出的成分所引起的生物学不良影响在公众中稳步增长。目的。在这项研究中,研究了人类牙龈成纤维细胞(HGF)中牙科树脂修复物中下列释放成分的细胞毒性和遗传毒性:四甘醇二甲基丙烯酸酯(TEEGDMA),新戊二醇二甲基丙烯酸酯(Neopen),二苯基碘化氯化物(DPIC),三苯基二苯乙烯(TPSB)和三苯膦(TPP)。方法。基于XTT的细胞生存力测定用于物质的细胞毒性筛选。 7-H2AX分析用于遗传毒性筛选。在7-H2AX分析中,HGF暴露于该物质6小时。诱导的病灶代表双DNA链断裂(DSB),它可以诱导组蛋白H_2AX的ATM依赖性磷酸化。由该物质引起的细胞死亡效应(凋亡和坏死)由同一研究者使用荧光显微镜目测。结果。所有测试的物质均导致HGF的剂量依赖性丧失活力。在XTT分析中发现,在ECso浓度下,这些物质之间的毒性排名如下(mM,平均值±SEM; n = 5):DPIC> Neopen> TPSB> TPP> TEEGDMA。当将HGF按以下顺序暴露于每种物质的EC_50浓度时,获得每个HGF细胞的DSB灶(平均值±SEM; n = 3):DPIC> Neopen> TPSB> TPP> TEEGDMA。在每种物质的ECso浓度下,发现80个HGF细胞中的多灶细胞(每个灶包含40个以上的灶)如下(平均值±SEM; n = 3):DPIC> Neopen> TPP> TPSB> TEEGDMA。每种物质在ECso浓度下的细胞凋亡顺序如下(平均值±SEM; n = 3):DPIC> Neopen> TPSB> TPP> TEEGDMA。 EC50浓度下,每种物质中包含的细胞坏死按以下顺序排列(平均值±SEM; n = 3):DPIC> Neopen> TPSB> TPP> TEEGDMA。结论。牙科树脂修复体中渗出的成分可诱导DNA生长激素(DSB)和细胞死亡。

著录项

  • 来源
    《Dental materials》 |2013年第9期|971-979|共9页
  • 作者单位

    Department of Operative/Restorative Dentistry, Periodontology and Pedodontics, Ludwig-Maximilians- University of Munich, Goethestr. 70, 80336 Munich, Germany and Walther-Straub-Institute of Pharmacology and Toxicology, Ludwig-Maximilians-University of Munich, Nussbaumstr. 26, 80336 Munich, Germany Walther-Straub-Institute of Pharmacology and Toxicology, Ludiuig-Maximilians-University of Munich, Nussbaumstr. 26, 80336 Munich, Germany;

    Department of Operative/Restorative Dentistry, Periodontology and Pedodontics, Ludurig-Maximilians-University of Munich, Goethestr. 70, 80336 Munich, Germany;

    Walther-Straub-Institute of Pharmacology and Toxicology, Ludiuig-Maximilians-University of Munich, Nussbaumstr. 26, 80336 Munich, Germany;

    KU Leuven BIOMAT, Department of Oral Health Science, University of Leuven, Kapucijnenvoer 7, B-3000 Leuven, Belgium;

    Walther-Straub-Institute of Pharmacology and Toxicology, Ludiuig-Maximilians-University of Munich, Nussbaumstr. 26, 80336 Munich, Germany;

    Walther-Straub-Institute of Pharmacology and Toxicology, Ludiuig-Maximilians-University of Munich, Nussbaumstr. 26, 80336 Munich, Germany;

    Department of Operative/Restorative Dentistry, Periodontology and Pedodontics, Ludurig-Maximilians-University of Munich, Goethestr. 70, 80336 Munich, Germany;

    Bundeswehr Institute of Radiobiology Affiliated to the University of Ulm, Neuherbergstr. 11, 80937 Munich, Germany;

    Department of Conservative Dentistry and Periodontology, Medical University Hannover, 30623 Hannover, Germany;

    Institute of Toxicology, Mainz University Medical Center, Obere Zahlbacherstr. 67, 55131 Mainz, Germany;

    Department of Organic Chemistry, Ludwig-Maximilians-University of Munich, Butenandtstr. 5-13, House F, 81377 Munich, Germany;

    Department of Operative/Restorative Dentistry, Periodontology and Pedodontics, Ludurig-Maximilians-University of Munich, Goethestr. 70, 80336 Munich, Germany Walther-Straub-Institute of Pharmacology and Toxicology, Ludiuig-Maximilians-University of Munich, Nussbaumstr. 26, 80336 Munich, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Composite; TEEGMA; TPP; TPSB; Neopen; DPIC; γ-H2AX assay; HGF;

    机译:综合;TEEGMA;TPP;TPSB;Neopen;DPIC;γ-H2AX分析;肝细胞生长因子;
  • 入库时间 2022-08-18 03:47:03

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