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Cytotoxicity of resin composites containing bioactive glass fillers

机译:含生物活性玻璃填料的树脂复合材料的细胞毒性

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摘要

Objective. To determine the in vitro cytotoxicity of dental composites containing bioactive glass fillers. Methods. Dental composites (50:50 Bis-GMA/TEGDMA resin: 72.5 wt% filler, 67.5%Sr-glass and 5% OX50) containing different concentrations (0, 5,10 and 15 wt%) of two sol-gel bioactive glasses, BAG65 (65 mole% SiO_2, 31mole% CaO, 4mole% P_2O_5) and BAG61 (3mole% F added) were evaluated for cytotoxicity using Alamar Blue assay. First, composite extracts were obtained from 7 day incubations of composites in cell culture medium at 37 ℃. Undif-ferentiated pulp cells (OD-21) were exposed to dilutions of the original extracts for 3,5, and 7 days. Then freshly cured composite disks were incubated with OD-21 cells (n = 5) for 2 days. Subsequently, fresh composite disks were incubated in culture medium at 37 ℃ for 7 days, and then the extracted disks were incubated with OD-21 cells for 2 days. Finally, fresh composites disks were light cured for 3, 5, and 20 s and incubated with OD-21 cells (n = 5) for 1, 3, 5, and 7 days. To verify that the three different curing modes produced different levels of degree of conversion (DC), the DC of each composite was determined by FTIR. Groups (n = 5) were compared with ANOVA/Tukey's (α ≤ 0.05). Results. Extracts from all composites significantly reduced cell viability until a dilution of 1:8 or lower, where the extract became equal to the control. All freshly-cured composites showed significantly reduced cell viability at two days. However, no reduction in cell viability was observed for any composite that had been previously soaked in media before exposure to the cells. Composites with reduced DC (3 s vs. 20 s cure), as verified by FTIR, showed significantly reduced cell viability. Significance. The results show that the composites, independent of composition, had equivalent potency in terms of reducing the viability of the cells in culture. Soaking the composites for 7 days before exposing them to the cells suggested that the "toxic" components had been extracted and the materials were no longer cytotoxic. The results demonstrate that the cytotoxicity of composites with and without BAG must predominantly be attributed to the release of residual monomers, and not to the presence of the BAG.
机译:目的。确定含有生物活性玻璃填料的牙科复合材料的体外细胞毒性。方法。牙科复合材料(50:50 Bis-GMA / TEGDMA树脂:72.5 wt%填料,67.5%Sr玻璃和5%OX50)包含两种浓度(0、5、10和15 wt%)的两种溶胶-凝胶生物活性玻璃,使用Alamar Blue分析评估BAG65(65摩尔%SiO_2、31摩尔%CaO,4摩尔%P_2O_5)和BAG61(添加3摩尔%F)的细胞毒性。首先,将复合物在37℃的细胞培养基中培养7天获得复合物提取物。未分化的果肉细胞(OD-21)暴露于原始提取物的稀释液中3.5、7天。然后将新鲜固化的复合盘与OD-21细胞(n = 5)孵育2天。随后,将新鲜的复合圆盘在37℃的培养基中孵育7天,然后将提取的圆盘与OD-21细胞孵育2天。最后,将新鲜的复合材料圆盘光固化3、5和20 s,并与OD-21细胞(n = 5)孵育1、3、5和7天。为了验证三种不同的固化方式产生不同水平的转化度(DC),通过FTIR确定每种复合材料的DC。将各组(n = 5)与ANOVA / Tukey's(α≤0.05)进行比较。结果。所有复合材料的提取物都会显着降低细胞活力,直到稀释度为1:8或更低,此时提取物变得与对照相同。所有新固化的复合材料在两天后均显示细胞活力显着降低。然而,对于在暴露于细胞之前先前浸泡在培养基中的任何复合物,未观察到细胞活力的降低。经FTIR验证,DC降低的复合材料(3 s比20 s固化)显示出明显的细胞活力降低。意义。结果表明,在降低培养细胞活力方面,该复合物与成分无关,具有同等效力。在将复合材料暴露于细胞之前将其浸泡7天,这表明已经提取了“有毒”成分,该材料不再具有细胞毒性。结果表明,含或不含BAG的复合材料的细胞毒性必须主要归因于残留单体的释放,而不是BAG的存在。

著录项

  • 来源
    《Dental materials》 |2015年第2期|195-203|共9页
  • 作者单位

    Division of Biomaterials and Biomechanics, Department of Restorative, School of Dentistry, Oregon Health Science University, Portland, OR, USA;

    Division of Biomaterials and Biomechanics, Department of Restorative, School of Dentistry, Oregon Health Science University, Portland, OR, USA;

    Midwestern University, Glendale, AZ, USA;

    Division of Biomaterials and Biomechanics, Department of Restorative, School of Dentistry, Oregon Health Science University, Portland, OR, USA;

    Division of Biomaterials and Biomechanics, Department of Restorative, School of Dentistry, Oregon Health Science University, Portland, OR, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cytotoxicity; Dental composites; Bioactive glass; Degree of Conversion; FTIR; Undifferentiated pulp cells (OD-21);

    机译:细胞毒性;牙科复合材料;生物活性玻璃转换度;FTIR;未分化的果肉细胞(OD-21);
  • 入库时间 2022-08-18 03:46:53

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