Intraspecific Differentiation in White Mistletoe (Viscum album L.) Using the Analysis of Intron Length Polymorphism of β-Tubulin Genes and the SSR Analysis

摘要

Based on the analysis of intron I length polymorphism of the beta-tubulin genes (TBP analysis), differences between two mistletoe subspecies (Viscum album ssp. austriacum (Wiesb.) and V. album ssp. album L.) have been shown. The possibility of using the Tubulin-Based Polymorphism (TBP) analysis for the sexual differentiation in mistletoe plants has also been demonstrated. We were successful in identifying particular white mistletoe genotypes using the simple sequence repeats (SSR) method. Application efficiency was comparatively assessed in both types of DNA markers, and the TBP analysis was shown to be more usable for differentiating between white mistletoe subspecies, whereas the SSR analysis is more suitable for studying genotypic variability within a particular subspecies.