Typing for brown trout LDH-C1* alleles together with microsatellites by automated sequencing

摘要

The eye-specific lactate dehydrogenase (LDH-C1) locus is a genetic marker of evolutionary and ecological interests for brown trout. We developed a new protocol to genotype the point mutation responsible for this polymorphism by automated sequencing. To that end, we designed and employed two primer pairs in a single polymerase chain reaction, each pair including one allele-specific primer. This new method is cheaper and more rapid than the previously published method that required a post-PCR restriction endonuclease digestion. Another attraction of the method is the possibility, presently tested, to co-amplify the LDH-C1 diagnostic region together with microsatellite loci.