Microsatellite markers isolated from polyploid Kielmeyera coriacea Mart. & Zucc. (Clusiaceae) from an enriched genomic library

摘要

Kielmeyera coriacea is a morphologically highly variable polyploid species, characteristic of the Cerrado, the savanna-like vegetation of the Central-Brazil. It was subject of various biological studies and, like others cerrado plants, this species suffers strong anthropogenic pressure. To access the genetic diversity of populations of this species, its taxonomic circumscriptions and to study supposed hybridization events, we have developed 11 polymorphic microsatellite loci from a CT/GT-enriched genomic library. The band pattern showed 12–32 bands per locus, and 2–8 bands per locus per individual. All microsatellites successfully amplified PCR products in K. grandiflora, the putative sister species, and all revealed similar multibanded pattern.