Quick DNA preparation for direct PCR in marine invertebrates and fishes

摘要

A modified protocol was developed for extracting DNA and direct PCR from tissues of marine invertebrates and finfishes. Protocol represented combination of classical lysis using a modified buffer system comprising KCl, Tris buffer and MgCl2. The DNA obtained was quantified and tested by mtDNA-PCR and RAPD. Test results indicated usefulness of this method in studies involving screening of large numbers of samples, such as evolutionary, forensic and population studies.