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Microsatellites for three distantly related genera in the Brassicaceae

机译:十字花科三个远缘属的微卫星

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摘要

Microsatellites are important genetic markers both in population genetics and for delimitation of closely related species. However, to develop microsatellites for each target organism is expensive and time consuming. In this study, we have therefore developed 65 new microsatellite primers for the species Draba nivalis and tested cross-species and cross-genus transfer success of these primers for two other genera in the Brassicaceae; Cardamine and Smelowskia. Furthermore, 15 previously developed microsatellites were tested for amplification in these three genera. The microsatellite markers that amplify across these genera may be useful for other genera in the Brassicaceae as well.
机译:微卫星既是种群遗传学中​​重要的遗传标志,又是界定密切相关物种的重要遗传标志。但是,为每种靶生物开发微卫星既昂贵又费时。因此,在这项研究中,我们开发了65种新的微卫星引物,用于Draba nivalis物种,并在十字花科的另外两个属中测试了这些引物的跨物种和跨属转移成功。豆蔻胺和Smelowskia。此外,在这三个属中测试了15个先前开发的微卫星的扩增能力。在这些属中扩增的微卫星标记也可能对十字花科的其他属有用。

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  • 来源
    《Conservation Genetics》 |2009年第3期|643-648|共6页
  • 作者单位

    National Centre for Biosystematics Natural History Museum University of Oslo P.O. Box 1172 Blindern 0318 Oslo Norway;

    National Centre for Biosystematics Natural History Museum University of Oslo P.O. Box 1172 Blindern 0318 Oslo Norway;

    Department of Botany University of British Columbia Vancouver BC Canada V6T 1Z4;

    National Centre for Biosystematics Natural History Museum University of Oslo P.O. Box 1172 Blindern 0318 Oslo Norway;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    SSR; Draba; Smelowskia; Cardamine; Cross-species transfer; Cross-genus transfer;

    机译:SSR;Draba;Smelowskia;Cardamine;跨物种转移;跨属转移;

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