Fast cleavage of 3', 5'-cyclic adenosine monophosphate and 3', 5'- cyclic guanosine monophosphate by lanthanides

摘要

Synthetic nucleases which rapidly cleave nucleic acids under mild conditions have many important potential applications ranging from the synthesis of custom-designed artificial restriction enzymes to the development of new antitumor drugs. Nonenzymatic hydrolysis of nucleic acids has been attracting increasing interest. However, the cleavage of nucleic acids by lanthanides has rarely been reported, and only Sumaoka et al, observed enormous hydrolysis of 3', 5' -cyclic adenosine monophosphate (cAMP) by cerium (III) hydroxide cluster. Hydrolysis of various kinds of 3', 5'-cyclic nucleotides by lanthanides has not been intensively studied. cAMP and 3', 5' -cyclic guanosine monophosphate (cGMP) are the key molecules which regulate response of cells against external stimulus, and various concentrations of cyclic nucleotides are related to the mechanism of the inci-dence of cardiovascular diseases. Here the cleavage of cAMP and cGMP by lanthanides was investigated by high-performance liquid chromatography (HPLC) and nuclear magnetic resonance(NMR), and the mechanism was further discussed. It is of momentous significance for seeking efficient and site-selective man-made nucleases and elucidating the biological effects of rare earths.