Molecular characterization of RAPD and SCAR marker linked to the frog-eye leaf spot resistance gene in soybean

摘要

Two fragments SCS3_620 and SCS3_580 of the co-dominant marker OPS03_620&580 that were linked to the resistance gene of soybean frog-eye leaf spot have been completely sequenced. A significant insertion of 30 bp is the main reason of the polymorphism between the two fragments. The results of Southern hybridization indicate that SCS3_620 derives from a signle- or low-copy Sequence and can be used as an RFLP probe. A co-dominant SCAR marker SCS3_620&580 has been Developed based on the sequences.