An experimental study on rat model of parkinsonizm by gene therapy

摘要

Conclusions In experimental gene therapy for PD, the pCMVTH is a useful vector for carrying TH gene. The lipofection is a practical technique for trans- ferring a target gene into eukaryotes and primary cul- tured muscle cells should be a good vehicle for DNA transfer and intracerebral grafting. Objective To induce significant improvement of motor abnormalities and striatal dopamine (DA) levels in rat model of Parkinson's disease (PD), by intrac- erebral grafting of the genetically modified muscle cells expressing tyrosine hydroxylase (TH).