首页> 外文期刊>Chinese Jouranl of Integrative Medicine >Effects of Astragalus membranaceus in Promoting T-helper Cell Type 1 Polarization and Interferon-γ Production by Up-regulating T-bet Expression in Patients with Asthma
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Effects of Astragalus membranaceus in Promoting T-helper Cell Type 1 Polarization and Interferon-γ Production by Up-regulating T-bet Expression in Patients with Asthma

机译:黄芪对哮喘患者T-bet表达的上调促进T辅助细胞1型极化和γ-干扰素产生的作用

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Objective: To explore the effect of Astragalus membranaceus (AM) on T-helper cell type 1 (Th1) specific transcription factor T-box expressed in T cells (T-bet) expression and Th1/Th2 equilibrium. Methods: The levels of T-bet mRNA in peripheral blood mononuclear cells (PBMCs) from 15 patients with asthma and 15 healthy subjects were determined by reverse transcription-polymerase chain reaction (RT-PCR). PBMCs in asthma patients were incubated with AM and then the concentration of interferon γ (IFN-γ) and interleukin-4 (IL-4) in the supernate before and after AM intervention were determined by ELISA. The numbers of CD4 + CCR3 + and CD4 + CCR5 + cells were counted by flow cytometry. Results: The expression of T-bet mRNA and the level of IFN-γ were lower, but level of serum IL-4 was higher in asthma patients when compared with those in healthy subjects respectively. After AM (60 μg/ml) intervention, the former two parameters raised and showed a positive correlation between them, while the level of IL-4 was decreased. The mean percentage of CD4 + CCR3 + cells in asthma patients was significantly higher but that of CD4 + CCR5 + cells was lower when compared with those in healthy subjects respectively. After AM intervention, the abnormal change in the two indexes was improved to certain extent, showing a reversing status of Th2 polarization. Conclusion: AM could increase the expression of T-bet mRNA and Th1 cytokines such as IFN-γ, and might reverse the Th2 predominant status in asthma patients.
机译:目的:探讨黄芪(AM)对T细胞表达的T辅助细胞1型(Th1)特异性转录因子T-box(T-bet)表达和Th1 / Th2平衡的影响。方法:采用逆转录-聚合酶链反应(RT-PCR)法检测15例哮喘患者和15例健康人外周血单个核细胞(PBMC)中T-bet mRNA的水平。将哮喘患者的PBMC与AM孵育,然后通过ELISA测定AM干预前后上清液中干扰素γ(IFN-γ)和白介素4(IL-4)的浓度。通过流式细胞术计数CD4 + CCR3 +和CD4 + CCR5 +细胞的数目。结果:与健康人相比,哮喘患者的T-bet mRNA表达和IFN-γ水平较低,但血清IL-4水平较高。 AM(60μg/ ml)干预后,前两个参数升高并显示正相关,而IL-4含量降低。与健康受试者相比,哮喘患者中CD4 + CCR3 +细胞的平均百分比显着较高,但CD4 + CCR5 +细胞的平均百分比较低。 AM干预后,两个指标的异常变化得到了一定程度的改善,呈现出Th2极化的反转状态。结论:AM可以增加哮喘患者T-bet mRNA和Th1细胞因子如IFN-γ的表达,并可能逆转Th2优势状态。

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