Novel Strong Cation-exchange Monolithic Column Using Poly(vinyl ester resin) as Stationary Phase Materials for Lysozyme Separation by HPLC

摘要

A novel cation-exchange polymeric monolith based on poly(vinyl ester resin) monolith was applied to separation in our present work. Poly- (vinyl ester resin) was as the monomer as well as the crosslinking agent in the polymerization which was induced by the initiator AIBN (2-methylpropropionitrile). 1-Hexadecanol was used as the porogen and methanol was used to make the 1-hexadecanol resolve easier. The short (1 cm) monolithic column was used to separate Lys from chicken egg white, and high resolution was obtained in this experiment. The results suggested that such kind of monolithic column could be used as a simple, cheap, effective method for the separation and purification of Lys. It is a good kind of stationary phase for HPLC.