Real-time PCR for rapidly detecting aniline-degrading bacteria in activated sludge

Takakazu Kayashima; Hisako Suzuki; Toshinari Maeda; Hiroaki I. Ogawa

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Real-time PCR for rapidly detecting aniline-degrading bacteria in activated sludge

机译：实时PCR快速检测活性污泥中的苯胺降解细菌

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摘要

We developed a detection method that uses quantitative real-time PCR (qPCR) and the TaqMan system to easily and rapidly assess the population of aniline-degrading bacteria in activated sludge prior to conducting a biodegradability test on a chemical compound. A primer and probe set for qPCR was designed by a multiple alignment of conserved amino acid sequences encoding the large (

机译：我们开发了一种检测方法，该方法使用定量实时PCR（qPCR）和TaqMan系统轻松快速地评估活性污泥中苯胺降解细菌的数量，然后再对化合物进行生物降解性测试。通过多重比对编码苯胺双加氧酶大（

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来源
《Chemosphere》 |2013年第9期|1338-1343|共6页
作者
Takakazu Kayashima; Hisako Suzuki; Toshinari Maeda; Hiroaki I. Ogawa;
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作者单位

Chemicals Evaluation and Research Institute, Kurume laboratory, 3-2-7 Miyanojin, Kurume-shi, Fukuoka 839-0801, Japan ,Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku,Kitakyushu 808-0196, Japan;

Chemicals Evaluation and Research Institute, Kurume laboratory, 3-2-7 Miyanojin, Kurume-shi, Fukuoka 839-0801, Japan;

Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku,Kitakyushu 808-0196, Japan;

Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku,Kitakyushu 808-0196, Japan;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
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关键词
aniline; biodegradation; aniline-degrading bacteria; activated sludge; real-time PCR;

机译：苯胺;生物降解苯胺降解细菌;活性污泥实时PCR;

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1. Real-time PCR assay for the simultaneous quantification of nitrifying and denitrifying bacteria in activated sludge [J] . Geets J, de Cooman M, Wittebolle L, Applied Microbiology and Biotechnology . 2007,第1期

机译：实时PCR分析同时定量分析活性污泥中硝化和反硝化细菌
2. Real-time PCR assay for the simultaneous quantification of nitrifying and denitrifying bacteria in activated sludge [J] . Joke Geets, Micha?l de Cooman, Lieven Wittebolle, Applied Microbiology and Biotechnology . 2007,第1期

机译：实时PCR分析同时定量分析活性污泥中硝化和反硝化细菌
3. Real-Time PCR Quantification of Ammonia-Oxidizing Bacteria (AOB): Solids Retention Time (SRT) Impacts during Activated Sludge Treatment of Industrial Wastewater [J] . David H.-W. Kuo, Kevin G. Robinson, Alice C. Layton, Environmental Engineering Science . 2006,第3期

机译：氨氧化细菌（AOB）的实时PCR定量：活性污泥处理工业废水过程中固体保留时间（SRT）的影响
4. A rapid method for genomic DNA extraction from activated sludge treating endosulfan manufacturing wastewater, and its ERIC-PCR fingerprint analysis [C] . Yu Fang-Bo, Wei Chen-Chen, Zhou Shan, 2011 International Conference on Electric Technology and Civil Engineering . 2011

机译：从活性污泥中提取基因组DNA的快速方法处理硫丹生产废水及其ERIC-PCR指纹分析
5. Rapid and Sensitive Detection of Pathogenic Bacteria in Chicken Products by Single Tube Nested Real-Time PCR [D] . Wu, Biyu. 2019

机译：单管嵌套实时PCR快速敏感地检测鸡产品的致病菌
6. Power Analysis for Real-Time PCR Quantification of Genes in Activated Sludge and Analysis of the Variability Introduced by DNA Extraction [O] . Hebe M. Dionisi, Gerda Harms, Alice C. Layton, 2003

机译：活性污泥中基因实时PCR定量的功效分析和DNA提取引入的变异性分析
7. Research on a Performance Comparison of Test Methods Evaluating Ready Biodegradability of Chemicals and a Development of Rapid Method for Detecting Aniline-degrading Bacteria [O] . 茅島孝和 2013

机译：评价化学药品易生物降解性的测试方法性能比较的研究和快速检测苯胺降解细菌的方法的发展

Real-time PCR for rapidly detecting aniline-degrading bacteria in activated sludge

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