Identification of quorum sensing signal AHLs synthases in Candidatus Jettenia caeni and their roles in anammox activity

摘要

Acyl-homoserine lactone (AHL)-based quorum sensing (QS) in the anaerobic ammonium oxidizing (anammox) consortia has attracted increasing attention. However, AHL synthase in anammox bacteria and the relationship between AHL synthetic genes and anammox activity are still not clear because anammox bacteria have not been isolated from the consortia. Two novel synthases of AHLs (JqsI-1 and JqsI-2), which are HdtS-type rather than the widely studied Luxl-type, were identified in anammox bacteria Candidatus Jettenia caeni and synthesized four AHLs. There was a correlation between AHL concentration, in situ transcriptional expression of the AHL synthase genes (jqsI-1 and jqsI-2) and genetic marker of anammox activity (hydrazine synthase gene, hzsA). And AHL add-back studies demonstrated that AHL influence the expression of hzsA to regulate anammox bacterial activity. This study provides insight into the QS communication pathway of anammox bacteria for wastewater treatment. (C) 2019 Published by Elsevier Ltd.