The ProteOn XPR36? Array System—High Throughput Kinetic Binding Analysis of Biomolecular Interactions

摘要

Surface plasmon resonance (SPR) technology is a central, widely used tool for kinetic studies of interactions between unlabeled biomolecules in real time. The ProteOn XPR36 protein interaction array system possesses all the qualities of high-level SPR biosensing technology combined with high-throughput and multiplexing capabilities. This system is based on the built-in orientation controlled multichannel module of the ProteOn XPR36 system, which allows parallel measurement of multiple binding interactions between as many as six protein pairs. This kind of multiplexing is done efficiently on the ProteOn XPR36 system using the innovative technique of One-shot KineticsTM, which allows simultaneous monitoring of multiple protein pair interactions. Here we demonstrate the capabilities of the ProteOn XPR36 for the measurements of a wide range of biomolecules interactions including proteins, DNA oligos, small molecules and antibodies and discuss its optimal mode of use.