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Differential expression of a cDNA clone in human liver versus hepatic cancer--highly homologous to aryl-dialkyl-phosphatase

机译:cDNA克隆在人肝和肝癌中的差异表达-与芳基-二烷基磷酸酶高度同源

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We applied the technique of mRNA differential display to normal liver tissue and hepatoma cell line Hep3B. One of the isolated cDNA clones was expressed in human normal liver tissue but not in the human hepatocarcinoma cell line. Northern Blot analysis confirmed that high level of mRNA was expressed in human normal liver tissue but the level was decreased in non-cancerous liver tissue from hepatoma patients. Low level or no expression was observed in human hepatoma tissue. One of these transcripts was about 1.8 kb in length. Southern Blot analysis showed that it was a single copy gene. We obtained a full length cDNA clone of 2,395 bp by screening human liver 5'-stretch plus cDNA library. Nucleotide sequence indicated that this clone was highly homologous to aryl-dialkyl-phosphatase and possessed two polymorphic sites. Aryl-dialkyl-phosphatase which has a prominent role in the metabolism of several toxic, synthetic compounds, may be potentially related to human hepatocarcinoma susceptibility. The biological significance of its differential expression in normal versus malignant tissue is discussed.
机译:我们将mRNA差异显示技术应用于正常肝组织和肝癌细胞系Hep3B。分离的cDNA克隆之一在人正常肝组织中表达,但不在人肝癌细胞系中表达。 Northern Blot分析证实,在正常肝组织中高水平的mRNA表达在肝癌患者的非癌性肝组织中降低了。在人肝癌组织中观察到低水平或没有表达。这些成绩单之一约为1.8 kb。 Southern印迹分析表明它是单拷贝基因。通过筛选人肝5'-stretch和cDNA文库,我们获得了2,395 bp的全长cDNA克隆。核苷酸序列表明该克隆与芳基-二烷基磷酸酶高度同源,并具有两个多态性位点。在几种有毒的合成化合物的代谢中起重要作用的芳基二烷基磷酸酶可能与人类肝癌的易感性有关。讨论了其在正常组织与恶性组织中差异表达的生物学意义。

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