Antisense oligonucleotide to insulin-like growth factor II induces apoptosis in human ovarian cancer AO cell line

摘要

The effectsof antisense oligonucleotide to insulin-like growth factor II (IGFII) to induce apoptosis in human ovarian cancer cells were evaluated. Antiproliferation ef- fects of antisense to IGFII in ovarian cancerr AO cells were determined by ~3H-thymidine incorporation. Apoptosis of the IGFII antisense-treated cells was quantitated by both nuclear condensation and flow cytometry after cells were stained with propidium iodide. IGFII antisense (4.5 μM) treatment of 48 h maximally inhibited proliferation of AO cells. More than 25/100 of IGFII antisense-treated cells (4.5 μM for 24 h) had undergone apoptosis, whereas less than 3/100 of the cells were apoptotic in either IGFII sense-treated cells or untreated cells.