NIH 3T3 cells malignantly transformed by mot-2 show inactivation and cytoplasmic sequestration of the p53 protein

摘要

In previous studies we have reported that a high level of expression of mot-2 protein results in malignant trans- formation of NIH 3T3 cells as analyzed by anchorage inde- pendent grwoth and nude mice assays[Kaul et al., Onco- gene, 17,907-11, 1998]. Mot-2 was found to interact with tumor suppressor protein p53. The transient overexpres- sion of mot-2 inhibitory to transcriptional activation function of p53[Wadhwa et al., J.Biol. Chem., 273, 29586- 91,1998). We demonstrate here that mot-2 transfected stable clone of NIH 3T3 that showed malignant properties indeed show inactivation of p53 function as assayed by exogeneous p53 dependent reporter.