PSMA mimotope isolated from phage displayed peptide library can induce PSMA specific immune response

摘要

Prostate-specific membrane antigen(PSMA)is a cell surface glycoprotein expressed predominantly in prostate secretory acinar epithelium and prostate cancer cells as well as in several extraprostatic tissues. Mouse mono- clonal antibody 4G5 specific tot he extracellular domain of PSMA was used to screen two phage displayed pep- tide libraries(9aa linear and 9aa cys library). Three 4G5- reactive phagotopes were identified. Sequence analysis of isolated clones demonstrated that the interaction motif "VDPA/SK" has high homology to 719-725 aa on PSMA.