Tropomyosin is localized in the nuclear matrix and chro- mosome scaffold of physarum polycephalum

摘要

The nuclei and chromosomes were isolated from plas- modia of Physarum polycephalum. The nuclear matrix and chromosome scaffold were obtained after the DNA and most of the proteins were extracted with Dnase I and 2 M NaCl. SDS-PAGE analyses revealed that the nuclear matrix and chromosome scaffold contained a 37 kD polypeptide which is equivalent to tropomyosin in molecular weight. Immunofluorescence observations upon slide preparations labelet with anti-tropomyosin antibody showed that the nuclear matrix and chromosome scaffold emanated bright fluorescenece, suggesting the presence of the antigen in them.