Substance P enhances the proliferation of rat anterior pituitary cells in vitro

摘要

Laboratory of Neuroendocrinology, Department of Physiology, The 4th Military Medical University, Xi'an 710032, China The undecapeptide substance P(SP) was shown to be intimately involved in both the structural and functional aspects of the anterior pituitary. Yet, in addition to its influences on hormonal secretion, SP may well possess more actions in this master gland. The present study was ftherefore aimed to investigate the effect of SP on the proliferation of rat anterior pituitary cells in primary culture. It was found that SP could dose-dependently increase the incorporation of tritiated thymidine (~3H-TdR) into cultured anterior pituitary cells. Other mammalian tachykinins such as neurokinin A and neurokinin B had similar effect but to varying degrees. The equipotent analogue of SP, Norleucine11 -SP(Nle~(11)-SP), also acted likewise, with its action antagonizable by spantide, a SP receptor blocker. To further characterize the nature of cells responsive to the challenge of SP, immunocytochemical staining against S-100 protein and some adenohypophyseal hormones was performed alone or plus autoradiography. The results showed that the percentage of S-100 protein-immunoreactive cells was apparently elevated by the ad-dtion of Nle~(11)-SP for 48 h, which indicates a preferential proliferation of folliculo-stellate cells under the regime. This was confirmed by increases in immunocytochemical or autoradiographical labelling indices of anterior pituitary cells treated similarly. Taken together, these results reveal that the trophic action of SP observed previously in other tissues is also present at least in cultured rat anterior pituitary cells, with responding cells being predominantly folliculo-stellate cells as typified by S-100 protein-immunoreactivity. Therefore, an intra-pituitary trophic action of SP in vivo could be anticipated.