Enzyme mass transfer coefficient in aqueous two-phase systems: modified spray extraction columns

摘要

The fractional dispersed phase hold-up and mass transfer coefficients were measured in modified spray columns of 50 mm i.d. using an aqueous two phase system of polyethylene glycol (PEG-4000)-sodium sulphate-buffer. The mass transfer coefficients were measured for amyloglucosidase and β-galactosidase. Both co-current and countercurrent modes of operation were investigated. The dispersed phase hold-up (∈_D) and the dispersed phase and the continuous phase mass transfer coefficients (k_da, k_Ca) increased with increasing dispersed phase velocity.