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Soft X-Ray Microscope with Zone Plate at UVSOR

机译:带XS防区板的软X射线显微镜

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A soft X-ray microscope with zone plates was set up at UVSOR [synchrotron radiation facility (750MeV, 200mA) at Institute for Molecular Science, Okazaki, Japan]. A 50nm line & space pattern could be resolved at λ = 3.2nm. With an environmental chamber (wet cell) using SiN windows, wet biological specimens, such as letus protoplasts, rabbit myofibrils, tubulin of COS cell and Deinococcus radiodurans strains, could be observed at λ = 2.5nm. In the present microscope, the numerical aperture of the condenser was much smaller than that of the objective. To adjust both the numerical apertures, an ellipsoidal condenser mirror system was tested, and preliminary result (an image of Cu mesh, 12.7μm pitch) was obtained.
机译:在UVSOR [日本冈崎分子科学研究所的同步辐射装置(750MeV,200mA)下,设置了带有分区板的软X射线显微镜。 50nm的线和间隔图案可以在λ= 3.2nm处解析。在使用SiN窗口的环境室(湿室)中,可以在λ= 2.5nm处观察到湿的生物标本,如letus原生质体,兔肌原纤维,COS细胞的微管蛋白和Deinococcus radiodurans菌株。在本显微镜中,聚光镜的数值孔径远小于物镜的数值孔径。为了调整这两个数值孔径,测试了一个椭圆形的聚光镜系统,并获得了初步结果(Cu网格图像,间距为12.7μm)。

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