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Laboratory test to assess sensitivity of bio-based earth materials to fungal growth

机译:实验室测试,评估生物基地球材料对真菌生长的敏感性

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The effect of molds present in buildings on the health of the occupants is a major issue hence, when a building material is developed, its sensitivity to microbial growth should be assessed. However, few studies have investigated fungal growth on bio-based building materials with the resources available in a laboratory specializing in materials. The objective of this paper is thus to propose a simple and efficient experimental method useful for construction materials laboratories, adapted from methods proposed in the literature. For this purpose, fungal growth was investigated under different environmental conditions on earth-based material with or without the addition of straw or hemp shiv. Samples were inoculated with a strain ofAspergillus brasiliensisand were incubated for 12 weeks at 76, 84 or 93% RH, and 30 °C or 20 °C. Reproducible results showed that earth-based materials were more sensitive to fungi when they were enriched in plant aggregates. Fungal development was observed on earth material containing plant aggregates after 4 weeks of exposure at 93% RH and 30 °C, whereas it was observed after 8 weeks on raw earth material under the same conditions. Additionally, the possibility of quantifying fungal development with increased sensitivity by using image analysis is proposed. Due to the growth of fungal species other thanA.brasiliensis, a natural inoculation approach is recommended. One of the conclusions is that liquid water is more favorable to mold growth than relative humidity alone. The addition of liquid water is thus recommended to accelerate the test.
机译:建筑物中存在的霉菌对居住者健康的影响是一个主要问题,因此,在开发建筑材料时,应评估其对微生物生长的敏感性。但是,很少有研究利用专门研究材料的实验室中可用的资源调查生物基建筑材料上的真菌生长。因此,本文的目的是根据文献中提出的方法,提出一种对建材实验室有用的简单有效的实验方法。为此,在不同环境条件下,在有或没有添加稻草或大麻的情况下,在土基材料上研究了真菌的生长。将样品用巴西曲霉菌株接种,并在76%,84%或93%RH,30°C或20°C下孵育12周。可重现的结果表明,当土壤中的物质富含植物聚集体时,它们对真菌更敏感。在93%RH和30°C下暴露4周后,在含有植物聚集体的土壤中观察到真菌生长,而在相同条件下,在原始地球上8周后观察到真菌生长。另外,提出了通过使用图像分析来定量提高真菌敏感性的可能性。由于巴西曲霉以外的真菌种类的生长,建议采用自然接种方法。结论之一是,液态水比单独的相对湿度更有利于霉菌的生长。因此建议添加液态水以加快测试速度。

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