Paired pulse analysis of ATP and noradrenaline release form sympathetic nerves of rat tail artery and mouse vas deferens: effects of K~+ channel blockers

摘要

The paired pulse stimulus paradigm - two pulses of equal strength delivered at variable interpulse intervals - was used to study the release of ATP and noradrenaline(NA) from post ganglionic sympathetic nerves of rat tail artery and mouse vas deferens. Excitatory junction currents(EJCs) were used to measure the release of ATP, and differential Pulse amperometry to measure that of NA. At interpulse intervals of 0.1-1 s paired pulse stimulation caused an increase in the size of the Second EJC, both in rat tail artery and mouse vas deferens. As the interpulse interval was increased To 10 s or more, the two EJCs became of equal size.