Invetigation of the functional role played by the chemokine monocyte chemoattractant protein-1 in interleukin-1-induced murine peritonitis

摘要

Intraperitoneal (i.p.) injection of murine recombinant IL-1β(mrIL-1β)produced a dose-dependent (0.5-50 ng) and time-related(0.5-2h) secretion of murine monocyte chemoattractant protein-1 (mMCP-1; 3-4 ng per cavity) in the lavage fluids. MCP-1 mRNA could also be detected in the cell pellets by reverse transcriptase-polymerase chain reaction(RT-PCR). MCP-1 levels were reduced by more than 90/100 by co-administration of IL-1 receptor antagonist (10 μg) (n =6, P<0.05). in contrast, an IL-1 mutant with low affinity for IL-1 receptor type I, termed yIL-1Βδ4(50 ng), produced only a modest release of the chemokine.