Inhibition by nitric oxide-releasing compounds of prostacyclin production in human endothelial cells

摘要

The effects of two chemically unrelated nitric oxide(NO)-releasing compounds were studied on prostacyclin production in lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells (HUVECs). The cells expressed cyclooxygenase-2(COX-2) protein and produced prostacyclin by NS- 398-sensitivve manner suggesting that prostacyclin production derives principally by COX-2 pathway. A novel NO-releasing oxatriazole derivative GEA 3175(1-30 μM) inhibited LPS-induced production Of prostacyclin in HUVECs in a dose-dependent manner being more potent than the earlier known NO- Donor S-nitroso-N-acetylpenicillamine(SNAP).