Investigation on the mechanisms involved in the central protective effect of amylin on gastric ulcers in rats

摘要

The mechanisms involved in the protective effect of amylin (administered into the brain ventricle, i.c.v.) on gastric ulcers induced by the oral administration of ethanol 50/100(EtOH), 1 ml/rat) or indomethacin (indomethacin, 20 mg kg~-1, at a dosing volume of 5 ml) were investigated in rats. The possible involvement of endogenous nitric oxide (NO) in the beneficial effect of amylin against EtOH-induced ulcers was examined. The inhibitor of NO-synthesis, N~G-nitro-L-arginine methyl ester (L-NAME, 70 mg kg~-1, s.c.) was injected 30 min before amylin (2.2 μg/rat, i.c.v.) followed by EtOH after a further 30 min. Rats were sacrified 1 h after EtOH. L-NAME completely removed the protective effect of amylin.