Inhibition of chemotaxis in A7r5 rat smooth muscle cells by a novel panel of inhibitors

摘要

Arginine-specific ADP-ribosyltransferase(ART) activity has been implicated in white cell chemotaxis. In this study, we examined the capacity of a panel of structurally unrelated inhibitors and pseudo- Substrates of ART to inhibit chemotaxis of A7r5 rat vascular smooth muscle cells in response to PDGF- BB. The IC_50 values for nicotinamide(12 mM) and novobiocin(165 μM)were similar to those observed For inhibition of chemotaxis by human polymorphonuclear neutrophil leucocytes(PMN), whereas Vitamins K_3 (IC_50 =22 μM) and K_1 (IC_50=95 μM)were less potent than previously described in PMNs.