Somatostatin displayed on filamentous phage as a receptor-specific agonist

摘要

In search of methods to identify bio-active ligands specific for G protein-coupled receptors with seven transmembrane spanning regions, we have developed a filamentous phage-based selection and functional screening method. First, methods for panning peptide phage on cells were established, using the hormone somatostatin As a model. Somatostatin was displayed on the surface of filamentous phage by cloning into phage(mid) Vectors and fusion to either pIII or PVIII viral coat proteins. Peptide displaying phage bound to a Polyclo9nal anti-somatostatin serum, and, more importantly. To several somatostatin receptor subtypes (Sst) expressed on transfected CHO-Kl cells, in a pattern which was dependent on the used display method.