Characterization of an atypical muscarinic cholinoceptor mediating contraction of the guinea-pig isolated uterus

摘要

In may smooth muscle tissues a minor M_3-muscarinic acetylcholine(mACh) receptor population mediates contraction, despite the presence of a larger M_2-mACh receptor population. However, this is not the case for guinea-pig uterus where radioligand binding and functional studies exclude a dominant role for M_3-mACh receptors. Using tissue from animals pre-treated with diethylstilboestrol, estimates of antagonist affinity were Made before and after selective alkylation procedures, together with estimates of agonist affinity to Characterise the mACh receptor population mediating carbachol-induced contraction of guinea-pig Isolated uterus.