Involvement of ET_A and ET_B receptors in the activation of phospholipase D by endothelins in cultured rat cortical astrocytes

摘要

This study was performed to characterize the receptor subtypes involved in the endothelin stimulation of phospholipase D(PLD) in rat cortical astrocytes in primary culture. PLD activity was determined by measuring the formation of [~32P]phosphatidylbutanol in [~32P]orthophosphate prelabelled cells stimulated in the presence of 25 mM butanol. The agonists enothelin-1 (ET-1), endothelin-3(ET-3), sarafotoxin 6c (S6c) and IRL 1620 elicited PLD activation in a concentration-dependent manner. The potencies of ET-1, ET-3 and S6c were Similar. The maximal effect evoked by the ET_B-preferring agonists, ET-3, S6c and IRL 1620, were Significantly lower than the maximal response to the non-selective agonist ET-1.